Journal: Translational Cancer Research
Article Title: 5MPN effectively targets PFKFB4 and inhibits the glucose metabolism process and invasion of glioblastoma
doi: 10.21037/tcr-2025-1740
Figure Lengend Snippet: KEGG and GO analysis of PFKFB4-related genes and the effect of 5MPN on glycolysis in GBM. (A,B) KEGG and GO analysis bubble plots of the top 50 genes most highly correlated with PFKFB4. (C) ATP production in different groups. (D) Oxygen consumption rate in different groups, with the vertical axis showing average fluorescence intensity. (E) ATP production detected by ATP fluorescence probe in different groups. Data in (C,D) are shown as the mean ± SD. n=3. *, P<0.05; ***, P<0.001, Student’s t -test. ATP, adenosine triphosphate; GBM, glioblastoma multiforme; GO, Gene Ontology; KEGG, Kyoto Encyclopedia of Genes and Genomes; NC, negative control; PFKFB4, phosphofructokinase-2/fructose-2,6-bisphosphatase 4; SD, standard deviation.
Article Snippet: ATP detection kit (Beyotime, S0026B) and ATP fluorescence probe (pCMV-Mito-AT1.03, Beyotime, D2606), as well as optical character recognition (OCR) detection kit (Elabscience, Wuhan, China; E-BC-F068), all of which were operated according to the strict instructions provided in the kit manuals.
Techniques: Fluorescence, Negative Control, Standard Deviation